Coding
Part:BBa_K3809001:Design
Designed by: Francisco Javier Castañeda Villagran Group: iGEM21_TecCEM (2021-08-13)
Novel Selection Cassette katE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1842
Illegal BamHI site found at 1752 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 111
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to modify some codons in order to meet the iGEM Biobrick Standards. We also had to modify some nucleotides to synthetize the sequence with IDT.
Source
The coding sequence for the katE gene comes from NCBI GenBank: M55161.1. It is the sequence from E.coli catalase HPII (katE) gene, complete cds. However, some codons were optimized in order to make it compatible with Biobrick standards.